Dual-Mode Hydrodynamic Railing and Arraying of Microparticles for Multi-Stage Signal Detection in Continuous Flow Biochemical Microprocessors

Authors: Ryan D. Sochol, Daniel Corbett, Sarah Hesse, William E.R. Krieger, Ki Tae Wolf, Minkyu Kim, Kosuke Iwai, Song Li, Luke P. Lee and Liwei Lin

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Continuous flow particulate-based microfluidic processors are in critical demand for emerging applications in chemistry and biology, such as point-of-care molecular diagnostics. Challenges remain, however, for accomplishing biochemical assays in which microparticle immobilization is desired or required during intermediate stages of fluidic reaction processes. Here we present a dual-mode microfluidic reactor that functions autonomously under continuous flow conditions to: (i) execute multi-stage particulate-based fluidic mixing routines, and (ii) array select numbers of microparticles during each reaction stage (e.g., for optical detection). We employ this methodology to detect the inflammatory cytokine, interferon-gamma (IFN-γ), via a six-stage aptamer-based sandwich assay.